PowerTrack™ SYBR Green Master Mix for qPCR from Thermo Fisher Scientific

Product Specs
ItemPowerTrack™ SYBR Green Master Mix for qPCR
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog NumberA46012 / A46109 / A46110 / A46111 / A46112 / A46113
ApplicationsGene expression analysis (2-Step*), DNA target detection, 2-Step* RNA detection, qPCR,
*RT purchased separately and performed in separate tube than amplification
TypeMaster Mix
Quantityvaries: 1 mL, 5 mL, 2 x 5 mL, 5 x 5 mL, 10 x 5 mL, 50 mL

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Dietary Vitamin D Mitigates Coronavirus-Induced Lung Inflammation and Damage in MiceVirusesDecember 15, 2023Gabriel Campolina-Silva, Ana Cláudia Dos Santos Pereira Andrade, Manoela Couto, Paloma G. Bittencourt-Silva, Celso M. Queiroz-Junior, Larisse de Souza B. Lacerda, Ian de Meira Chaves, Leonardo C. de Oliveira, Fernanda Martins Marim, Cleida A. Oliveira, et al.7500 Fast Real-Time PCR System (Thermo ABI, Waltham, MA, USA) using a 10 μL PowerTrack™ SYBR™ Green Master Mix (Catalog No. A46109, Applied Biosystems, Waltham, MA, USA), with 2 ng of cDNA and 0.5 μM of both forward and reverse primers

(2) Loss of Slc35a2 alters development of the mouse cerebral cortexbioRxivDecember 1, 2023Soad Elziny, Sahibjot Sran, Hyojung Yoon, Rachel R. Corrigan, John Page, Amanda Ringland, Anna Lanier, Sara Lapidus, James Foreman, Erin L. Heinzen, et al.Pro 6 system (Thermo Scientific, A43180) using PowerTrack SYBR Green Master Mix (Applied Biosystems, A46109). Pre-designed primers for Slc35a2 (Mm.PT.58.29074836) and Gapdh (Mm.PT.39a.1) were obtained from Integrated

(3) Niraparib restrains prostate cancer cell proliferation and metastasis and tumor growth in mice by regulating the lncRNA MEG3/miR-181-5p/GATA6 pathwayPeerJNovember 29, 2023Ji Cheng, Yi Sun, Huacai Zhao, Wei Ren, Dan Gao, Zhigang Wang, Wei Lv, Qingchuan Dongmin, 37 °C for 5 min and 42 °C for 60 min. RT-qPCR analysis was performed using SYBR Green Master Mix (A46110; Applied Biosystems, Foster City, CA, USA) under the following reaction conditions: 95 °C for 10 min,

(4) Combinatorial CRISPR screens and lectin microarrays identify novel glycosylation regulatorsbioRxivNovember 28, 2023C Kimberly Tsui, Nicholas Twells, Emma Doan, Janiya Brooks, Ayodeji Kulepa, Brant Webster, Lara K Mahal, Andrew Dillinaccording to manufacturer’s instructions. SYBR Green master mix (Applied Biosystem, catalog number A46109) and primers listed below were used to set up qPCR reactions and analyzed on QuantStudio 6 Flex. Flow cytometry analysis

(5) Epigenetically Downregulated Breast Cancer Gene 2 through Acetyltransferase Lysine Acetyltransferase 2B Increases the Sensitivity of Colorectal Cancer to OlaparibCancers (Basel)November 25, 2023Siche Chen, Heike Allgayeranalysis, and Q-PCR was performed using the SYBR Green PCR master mix (Thermo Fisher, Berlin, Germany, A46012). Cycle numbers were normalized to those of β-actin (QIAGEN GmbH, Düsseldorf, Germany, QT00095431).

(6) Intranasal Liposomal Formulation of Spike Protein Adjuvanted with CpG Protects and Boosts Heterologous Immunity of hACE2 Transgenic Mice to SARS-CoV-2 InfectionVaccines (Basel)November 20, 2023Momtchilo Russo, Maria Cássia Mendes-Corrêa, Bruna B. Lins, Victor Kersten, Paulo C. A. Pernambuco Filho, Toni Ricardo Martins, Tânia Regina Tozetto-Mendoza, Lucy Santos Vilas Boas, Brisa Moreira Gomes, Livia Mendonça Munhoz Dati, et al.performed using SYBR green amplifications (PowerTrack SYBR Green Master Mix, Thermo Fisher Scientific, cat# A46109, Waltham, MA, USA). Reactions consisted of 10 μL of SYBR mix, 2 μL of cDNA, 1 μL (10 μM) of each primer,

(7) Evaluation of Neural Regulation and Microglial Responses to Brain Injury in Larval Zebrafish Exposed to Perfluorooctane SulfonateEnvironmental Health PerspectivesNovember 15, 2023Shannon E. Paquette, Nathan R. Martin, April Rodd, Katherine E. Manz, Eden Allen, Manuel Camarillo, Hannah I. Weller, Kurt Pennell, Jessica S. PlavickiFisher Scientific) or custom primers with Power Track SYBR Green Master Mix (Thermo Fisher, Cat. No. A46012). See list of probes and primers for qRT-PCR and genotyping in Table S2. Genotyping irf8 Mutants irf8st96/st96

(8) USP39 interacts with SIRT7 to promote cervical squamous cell carcinoma by modulating autophagy and oxidative stress via FOXM1Journal of Translational MedicineNovember 13, 2023Juanpeng Yu, Shuai Yuan, Jinglin Song, Shengsheng YuChina) was used for RNA reverse transcription. qRT-PCR was conducted with a SYBR Green PCR Master Mix (A46012, Thermo Fisher). Relative expression of SIRT7, USP39 and FOXM1 was analyzed using the 2−ΔΔCt method

(9) Multilayered regulation of developmentally programmed pre-anthesis tip degeneration of the barley inflorescenceThe Plant CellOctober 30, 2023Nandhakumar Shanmugaraj, Jeyaraman Rajaraman, Sandip Kale, Roop Kamal, Yongyu Huang, Venkatasubbu Thirulogachandar, Adriana Garibay-Hernández, Nagaveni Budhagatapalli, Yudelsy Antonia Tandron Moya, Mohammed R Hajirezaei, et al.Kit (Invitrogen, 18080-051). qPCR was performed using power SYBR Green PCR Master Mix (Thermo Fisher, A46112) on an ABI prism 7900HT sequence detection system (Applied Biosystems). The relative gene expression

(10) Seipin overexpression attenuates cerebral ischemia‐reperfusion injury via preventing apoptosis and autophagyBrain and BehaviorOctober 27, 2023Xiaoxi Zhu, Xiaoqiong An, Ming Chen, Dongfen Guo, Peng Xie, Bi Wang, Zhi Huang, Wenfeng Yumin. The amplification reaction was conducted using SYBR Green PCR Master Mix (Applied Biosystems, A46012). The reaction system was prepared with 10 μL of SYBR Mixture, 1 μL of PCR Forward Primer (10 μM), 1

(11) Phospholipid isotope tracing reveals β-catenin-driven suppression of phosphatidylcholine metabolism in hepatocellular carcinomabioRxivOctober 21, 2023Chad VanSant-Webb, Hayden K. Low, Junko Kuramoto, Claire E. Stanley, Hantao Qiang, Audrey Su, Alexis N. Ross, Chad G. Cooper, James E. Cox, Scott A. Summers, et al.μM forward primer, 2.5% 100 μM reverse primer, and 62.5% PowerTrack SYBR Green Master Mix (Thermo, A46109) in RNase-free water. Master mixes were combined 4:1 with the cDNA reactions and plated in duplicate.

(12) Loss of the KN Motif and AnKyrin Repeat Domain 1 (KANK1) Leads to Lymphoid Compartment Dysregulation in Murine ModelGenes (Basel)October 16, 2023Marwa Almosailleakh, Sofia Bentivegna, Samuele Narcisi, Sébasitien J. Benquet, Linn Gillberg, Carmen P. Montaño-Almendras, Simonas Savickas, Erwin M. Schoof, Amelie Wegener, Hérve Luche, et al.CA, USA). Quantitative PCR was performed using PowerTrackTM SYBR Green Master Mix for qPCR (Cat. no. A46109, ThermoFisher Scientific, Waltham, MA, USA) and an ABI prism 7500 sequence instrument. Ct values were

(13) Topoisomerase Inhibitors Increase Episomal DNA Expression by Inducing the Integration of Episomal DNA in Hepatic CellsPharmaceuticsOctober 13, 2023Andoni Gómez-Moreno, Enara San Sebastian, Jennifer Moya, Pilar Gomollón-Zueco, Sergio Isola, África Vales, Gloria González-Aseguinolaza, Carmen Unzu, Urtzi Garaigorta4319983, Applied Biosystems, Waltham, MA, USA) and the PowerTrackTM SYBR Green qPCR Master Mix (cat. nº: A46109, ThermoFisher Scientific, Waltham, MA, USA). Next, 10-fold serial dilutions of plasmids containing each

(14) Modulation of Influenza A virus NS1 expression reveals prioritization of host response antagonism at single-cell resolutionFrontiers in MicrobiologyOctober 9, 2023Qing Yang, Anna E. Elz, Maryline Panis, Ting Liu, Benjamin E. Nilsson-Payant, Daniel Blanco-MelocDNA was then diluted and used for qPCR analysis via PowerTrack SYBR Green master mix (Thermo Fisher. A46109). The qPCR was performed using BioRad CFX96 machine for 40 cycles, and the Ct values were used for relative

(15) Diverse evolutionary pathways challenge the use of collateral sensitivity as a strategy to suppress resistanceeLifeSeptember 22, 2023Rebecca E K Mandt, Madeline R Luth, Mark A Tye, Ralph Mazitschek, Sabine Ottilie, Elizabeth A Winzeler, Maria Jose Lafuente-Monasterio, Francisco Javier Gamo, Dyann F Wirth, Amanda K Lukens,Zymo Research,Cat No: R1200-25, Commercial assay or kit,SYBR Green Master Mix,Applied Biosystems,Cat No: A46109, "Chemical compound, drug",IDI-6273,ChemDiv,ChemDiv4861-0080, "Chemical compound, drug",Genz669178,"Genzyme,

(16) Sestrin2 protects against hypoxic nerve injury by regulating mitophagy through SESN2/AMPK pathwayFrontiers in Molecular BiosciencesSeptember 21, 2023Cunyao Pan, Chongyi Ai, Lanlan Liang, Baoyi Zhang, Qionglin Li, Lingling Pu, Zirou Wang, Weili Liu, Zhaoli Chen, Hui Liu, et al.step, which was carried out using a SYBR Green Real-time PCR Master Mix (Thermo Fisher Scientific, A46109), and Actb (encoding β-actin) as the internal control. The 2−ΔΔCT method was used to analyze the results

(17) Regulation of sexually dimorphic placental adaptation in LPS exposure-induced intrauterine growth restrictionMolecular MedicineSeptember 18, 2023Da Som Jeong, Ji-Yeon Lee, Myoung Hee Kim, Ji Hoon Oh(RT-qPCR), cDNA was amplified using SYBR green PCR Master Mix (Applied Biosystems, California, USA, A46109) on an ABI7300 real-time PCR system (Applied Biosystems) according to the manufacturer’s instructions.

(18) Reovirus infection of tumor cells reduces the expression of NKG2D ligands, leading to impaired NK-cell cytotoxicity and functionalityFrontiers in ImmunologySeptember 11, 2023Raghad Khaleafi, Jelena Zeleznjak, Sapir Cordela, Shani Drucker, Tihana Lenac Rovis, Stipan Jonjic, Yotam Bar-On1708890). Finally, qPCR was performed using the PowerTrack SYBR Green Master Mix (Thermo Fisher Scientific, A46109). All kits were used according to the manufacturer’s instructions. The qPCR reaction was read using

(19) Purinergic neurotransmission receptor P2X4 silencing alleviates intracerebral hemorrhage-induced neuroinflammation by blocking the NLRP1/Caspase-1 pathwayScientific ReportsAugust 31, 2023Yuanshui Wu, Xiaoli Huang, Le Yang, Yuanjie LiuScientific A48571). Afterward, real-time PCR was performed with SYBR Green (Thermo Fisher Scientific, A46110). Different gene expressions were calculated using the 2−∆∆Ct method and normalized to β-actin. Primer

(20) DNA-PK is activated by SIRT2 deacetylation to promote DNA double-strand break repair by non-homologous end joiningNucleic Acids ResearchAugust 25, 2023PamelaSara E Head, Priya Kapoor-Vazirani, Nagaraju P Nagaraju, Hui Zhang, Sandip K Rath, Nho C Luong, Ramona Haji-Seyed-Javadi, Fatmata Sesay, Shi-Ya Wang, Duc M Duong, et al.samples was extracted and subjected to quantitative real-time PCR (PowerTrack™ SYBR Green Master Mix, A46012; and PCR 7500 Fast Real-Time PCR system, ThermoFisher) using the following primers at the Fok1-induced

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2/7/2024Superb SYBR Green Master Mix From Thermo Fisher Scientific Abhijit NirwaneWe study the CNS basement membrane and blood-brain barrier. Specifically the role of different CNS cell types in BBB maintenance.